mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri
Identifieur interne : 001443 ( Main/Exploration ); précédent : 001442; suivant : 001444mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri
Auteurs : M. Golmohammadi [Espagne] ; P. Llop [Espagne] ; G. Scuderi [Italie] ; I. Gell [Espagne] ; J. H. Graham [États-Unis] ; J. Cubero [Espagne]Source :
- Plant Pathology [ 0032-0862 ] ; 2012-06.
English descriptors
Abstract
The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445‐bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.
Url:
DOI: 10.1111/j.1365-3059.2011.02526.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001936
- to stream Istex, to step Curation: 001935
- to stream Istex, to step Checkpoint: 000113
- to stream Main, to step Merge: 001458
- to stream Main, to step Curation: 001443
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title><author><name sortKey="Golmohammadi, M" sort="Golmohammadi, M" uniqKey="Golmohammadi M" first="M." last="Golmohammadi">M. Golmohammadi</name></author><author><name sortKey="Llop, P" sort="Llop, P" uniqKey="Llop P" first="P." last="Llop">P. Llop</name></author><author><name sortKey="Scuderi, G" sort="Scuderi, G" uniqKey="Scuderi G" first="G." last="Scuderi">G. Scuderi</name></author><author><name sortKey="Gell, I" sort="Gell, I" uniqKey="Gell I" first="I." last="Gell">I. Gell</name></author><author><name sortKey="Graham, J H" sort="Graham, J H" uniqKey="Graham J" first="J. H." last="Graham">J. H. Graham</name></author><author><name sortKey="Cubero, J" sort="Cubero, J" uniqKey="Cubero J" first="J." last="Cubero">J. Cubero</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3360682130BACCA3166C8B91442BE69FF5F117E1</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1111/j.1365-3059.2011.02526.x</idno><idno type="url">https://api.istex.fr/document/3360682130BACCA3166C8B91442BE69FF5F117E1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001936</idno><idno type="wicri:Area/Istex/Curation">001935</idno><idno type="wicri:Area/Istex/Checkpoint">000113</idno><idno type="wicri:doubleKey">0032-0862:2012:Golmohammadi M:mrna:from:selected</idno><idno type="wicri:Area/Main/Merge">001458</idno><idno type="wicri:Area/Main/Curation">001443</idno><idno type="wicri:Area/Main/Exploration">001443</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title><author><name sortKey="Golmohammadi, M" sort="Golmohammadi, M" uniqKey="Golmohammadi M" first="M." last="Golmohammadi">M. Golmohammadi</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Llop, P" sort="Llop, P" uniqKey="Llop P" first="P." last="Llop">P. Llop</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Scuderi, G" sort="Scuderi, G" uniqKey="Scuderi G" first="G." last="Scuderi">G. Scuderi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Phytosanitary Sciences and Technologies (DISTEF), Plant Pathology section, University of Catania, 95123 Catania</wicri:regionArea><wicri:noRegion>95123 Catania</wicri:noRegion></affiliation></author><author><name sortKey="Gell, I" sort="Gell, I" uniqKey="Gell I" first="I." last="Gell">I. Gell</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Graham, J H" sort="Graham, J H" uniqKey="Graham J" first="J. H." last="Graham">J. H. Graham</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Florida, Citrus Research and Education Center (CREC) 700 Experiment Station Road, Lake Alfred, FL 33850‐2299</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Cubero, J" sort="Cubero, J" uniqKey="Cubero J" first="J." last="Cubero">J. Cubero</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Pathology</title><idno type="ISSN">0032-0862</idno><idno type="eISSN">1365-3059</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06">2012-06</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="479">479</biblScope><biblScope unit="page" to="488">488</biblScope></imprint><idno type="ISSN">0032-0862</idno></series><idno type="istex">3360682130BACCA3166C8B91442BE69FF5F117E1</idno><idno type="DOI">10.1111/j.1365-3059.2011.02526.x</idno><idno type="ArticleID">PPA2526</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0862</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus</term><term>bacterial survival</term><term>citrus bacterial canker</term><term>real‐time PCR</term><term>viable bacteria</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445‐bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>Italie</li><li>États-Unis</li></country><region><li>Communauté de Madrid</li><li>Floride</li></region><settlement><li>Madrid</li></settlement></list><tree><country name="Espagne"><noRegion><name sortKey="Golmohammadi, M" sort="Golmohammadi, M" uniqKey="Golmohammadi M" first="M." last="Golmohammadi">M. Golmohammadi</name></noRegion><name sortKey="Cubero, J" sort="Cubero, J" uniqKey="Cubero J" first="J." last="Cubero">J. Cubero</name><name sortKey="Gell, I" sort="Gell, I" uniqKey="Gell I" first="I." last="Gell">I. Gell</name><name sortKey="Llop, P" sort="Llop, P" uniqKey="Llop P" first="P." last="Llop">P. Llop</name></country><country name="Italie"><noRegion><name sortKey="Scuderi, G" sort="Scuderi, G" uniqKey="Scuderi G" first="G." last="Scuderi">G. Scuderi</name></noRegion></country><country name="États-Unis"><region name="Floride"><name sortKey="Graham, J H" sort="Graham, J H" uniqKey="Graham J" first="J. H." last="Graham">J. H. Graham</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001443 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001443 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:3360682130BACCA3166C8B91442BE69FF5F117E1
|texte= mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri
}}
| This area was generated with Dilib version V0.6.25. |  |